Journal: PLoS ONE

Article Title: Assessing the binding properties of the anti-PD-1 antibody landscape using label-free biosensors

doi: 10.1371/journal.pone.0229206

Figure Lengend Snippet: (A) Scatter plots reporting the mean k a , k d , and K D values for LSA data produced on different chip types, HC-30M (X axis) compared with CMD-P (orange) and CMD-200M (grey). The mean values were calculated from 8–12 measurements (spots) per mAb. (B) Scatter plots reporting single measurements of k a , k d , and K D for Biacore 8K data produced on different chip types, C1 (X axis) compared with CMD-P (orange), CM5 (green) and CMD-200M (grey). (C) Benchmarking LSA-CMD-P data against Biacore 8K-C1 data, where the LSA data represent the mean (symbol) ± stdev (Y-axis error bars) for 8–12 replicate measurements (spots) per mAb. In each plot, a perfect correlation is indicated by the dashed diagonal line. The pink shaded areas define a two-fold deviation from perfect correlation. Panels A and C show data for 33 mAbs, whereas panel B shows data for 29 mAbs. Extreme outliers or data with poor fits to a 1:1 biding model were excluded.

Article Snippet: High-throughput SPR capture kinetic experiments were performed on Carterra’s LSA system equipped with Xantec CMD-P (flat), HC-30M (nearly flat), and CMD-200M (3D-hydrogel) chip types.

Techniques: Produced

Journal: PLoS ONE

Article Title: Assessing the binding properties of the anti-PD-1 antibody landscape using label-free biosensors

doi: 10.1371/journal.pone.0229206

Figure Lengend Snippet: Representative solution affinity curves for mAb30 via (A) KinExA (n-curve analysis) and (B) MSD (single curve analysis). (C) Correlation between KinExA (Y-axis) and MSD (X-axis) for 13 mAbs. (D) Correlation between SPR (Y-axis, average K D for Biacore 8K) for 27 mAbs on 3D-hydrogel (CM5 and CMD200 chips, grey) or flat chip (C1 and CMD-P, orange) and MSD (X-axis). The slope of the trendlines indicate average deviation from a perfect correlation for each chip type. Data for antibodies (mAb09, mAb18 and mAb29) measured at the off-rate limit of detection (< 4.27 x 10 −5 s -1 ) for the SPR experiments where the correlation does not conform are indicated with open circles, and not included in the trendline calculation. Data from mAb27 and mAb33 were excluded from this plot due to poor fits to a 1:1 binding model (see the associated ).

Article Snippet: High-throughput SPR capture kinetic experiments were performed on Carterra’s LSA system equipped with Xantec CMD-P (flat), HC-30M (nearly flat), and CMD-200M (3D-hydrogel) chip types.

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Assessing the binding properties of the anti-PD-1 antibody landscape using label-free biosensors

doi: 10.1371/journal.pone.0229206

Figure Lengend Snippet: Direct comparison of the apparent affinities ( K D values, nM) determined by solution methods (MSD and KinExA) and SPR (Biacore 8K) on flat chips (average of C1 and CMD-P) and 3D-hydrogels (average of CM5 and CMD200M). Thirty-two mAbs are included for this comparison and are ranked by their MSD/SPR affinity correlation (best to worst). ND = not determined; NB = non-binder in the assay; Bold = k d -limited in the Biacore SPR capture-based assay. See Fig 4B .

Article Snippet: High-throughput SPR capture kinetic experiments were performed on Carterra’s LSA system equipped with Xantec CMD-P (flat), HC-30M (nearly flat), and CMD-200M (3D-hydrogel) chip types.

Techniques: